mouse brain coronal frozen sections  (AMS Biotechnology)

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Rapid actions of anti-Müllerian hormone in regulating synaptic transmission and long-term synaptic plasticity in the hippocampus

doi: 10.1096/fj.201902217R

Figure Lengend Snippet: Protein localization of anti-Müllerian hormone (Amh) and the ligand-specific type II receptor (Amhr2) in the mouse hippocampus. Coronal sections were fluorescent-labeled for Amh (red; A,E), Amhr2 (green; B,F) and DAPI (nuclear staining; blue; C,G). A high magnification image of the boxed area is shown in the top-left insert for each panel. A,E) Amh-positive staining was detected in the Cornu Ammonis (CA) 1 and CA3 regions. B,F) Amhr2-positive staining was detected in the CA1 and CA3 regions. D,H) Amh- and Amhr2-positive staining was co-localized in cell bodies and dendrites (arrows). Scale bar = 40 μm for panels A-H and 10 μm for inserts.

Article Snippet: Amh and Amhr2 protein expression was further localized in the hippocampus using immunofluorescence staining on mouse brain coronal frozen sections (n = 3 males or females; MF-201–08; Zyagen, San Diego, CA, USA), as previously described ( 6 ).

Techniques: Labeling, Staining

Journal: Analytical chemistry

Article Title: Workflow for Combined Proteomics and Glycomics Profiling from Histological Tissues

doi: 10.1021/ac5022216

Figure Lengend Snippet: Consecutive enzyme digestion to fresh frozen and fixed mouse brain tissues. Abundances of HA depolymerization products (A), abundances of CS disaccharides (B), abundances of HS disaccharides (C,D), absolute abundances of native N-glycans (E), relative abundances of native N-glycans (F) and abundances of ACTB_MOUSE, ATPA_MOUSE and AT1A3_MOUSE proteins (G). Panel C shows HS disaccharide abundance normalized to internal standard and protein content, whereas panel D represents the % abundance of the disaccharide structures normalized to the summed intensity of all disaccharide structures of the sample. Error bars show the standard error where applicable. Hyaluronic acid depolymerization products (A) and heparan sulfate disaccharides (C and D) could only be detected in the combined extracts of serial sections in mouse brain samples; therefore there are no error bars in case of these samples. Panels E and F represent results of three triplicate measurements of the combined N-glycan extracts of serial sections. The following nomenclature is used for the HS disaccharides.45 The unsaturated uronic acid is marked by a “D”, whereas the glucosamine is marked by an “A” if it is unsulfated and by an “S” if it is N-sulfated. The number after “D” denotes the nonreducing end O-sulfation (0 if it is nonsulfated and 2 if it is 2-O-sulfated), while the number after “A” or “S” denotes hexosamine O-sulfation (0 if it is nonsulfated and 6 if it is 6-O-sulfated).

Article Snippet: Materials Fresh frozen bovine cerebral cortex slides containing two 15 μ m thick sections, FFPE bovine cerebral cortex slides (5 μ m thick, 4 sections per slide) and fresh frozen mouse caudate putamen brain coronal sections (10 μ m) were purchased from Zyagen (San Diego, CA).

Techniques: Glycoproteomics

Journal: Analytical chemistry

Article Title: Workflow for Combined Proteomics and Glycomics Profiling from Histological Tissues

doi: 10.1021/ac5022216

Figure Lengend Snippet: Consecutive enzyme digestion to fresh frozen and fixed mouse brain tissues. Abundances of HA depolymerization products (A), abundances of CS disaccharides (B), abundances of HS disaccharides (C,D), absolute abundances of native N-glycans (E), relative abundances of native N-glycans (F) and abundances of ACTB_MOUSE, ATPA_MOUSE and AT1A3_MOUSE proteins (G). Panel C shows HS disaccharide abundance normalized to internal standard and protein content, whereas panel D represents the % abundance of the disaccharide structures normalized to the summed intensity of all disaccharide structures of the sample. Error bars show the standard error where applicable. Hyaluronic acid depolymerization products (A) and heparan sulfate disaccharides (C and D) could only be detected in the combined extracts of serial sections in mouse brain samples; therefore there are no error bars in case of these samples. Panels E and F represent results of three triplicate measurements of the combined N-glycan extracts of serial sections. The following nomenclature is used for the HS disaccharides.45 The unsaturated uronic acid is marked by a “D”, whereas the glucosamine is marked by an “A” if it is unsulfated and by an “S” if it is N-sulfated. The number after “D” denotes the nonreducing end O-sulfation (0 if it is nonsulfated and 2 if it is 2-O-sulfated), while the number after “A” or “S” denotes hexosamine O-sulfation (0 if it is nonsulfated and 6 if it is 6-O-sulfated).

Article Snippet: Materials Fresh frozen bovine cerebral cortex slides containing two 15 μ m thick sections, FFPE bovine cerebral cortex slides (5 μ m thick, 4 sections per slide) and fresh frozen mouse caudate putamen brain coronal sections (10 μ m) were purchased from Zyagen (San Diego, CA).

Techniques: